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bp0005rdr9 | Reproductive Technology | REDR2002

Morphological assessment of preimplantation embryo quality in cattle

Maddox-Hyttel P , Gjørret JO , Vajta G , Alexopoulos NI , Lewis I , Trounson A , Viuff D , Laurincik J , Müller M , Tveden-Nyborg P , Thomsen PD

The extensive use of embryo technologies has emphasized the need for assessing embryo quality by morphological techniques, such as transmission electron microscopy, immunocytochemistry for confocal laser scanning microscopy and fluorescence in situ hybridization. By a combination of these techniques, it has been possible to demonstrate: (i) that rRNA gene activation, as monitored by embryonic nucleolar development, is comparable in bovine embryos developed in vivo...